Welcome

Every two years, the van LeeuwenHoek Centre for Advanced Microscopy (LCAM) organises a FEBS advanced practical course on fluorescence microscopy for PhD students, postdoc and other interested scientists. This year the topic of the course will be focused on visualising cellular dynamics:

The ability of cells to perceive and correctly respond to their environment is the basis of development, tissue repair, and immunity as well as normal tissue homeostasis. Errors in the interplay, dynamics or localization of the involved molecules are responsible for many diseases such as cancer, autoimmunity, and diabetes. By increasing the understanding of the complex interplay between these various components and their dynamics over time the related diseases can be treated more effectively. Because of the recent technological revolution in advanced light microscopy (i.e. functional imaging of molecules, development of caged compounds and biosensors) it is now possible to directly monitor the dynamics of these  (single) molecules and numerous biosensors in living cells.

Several of these new microscopy techniques are available but their application remains limited because most biologists have never been introduced to these technologies. This practical advanced course will be organized to provide participants the theoretical background and give hands-on experience of state-of-the-art microscopy techniques.

During this course, you will learn different microscopy techniques for studying biological processes in living cells. The course will cover the following techniques and tools:

Confocal microscopy, Total Internal Reflection Microscopy (TIRF), Förster resonance energy transfer (FRET), Fluorescence lifetime imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), Fluorescence recovery after Photobleaching (FRAP), Optogenetics, Data-analysis & Biosensors.

If you have questions, please do not hesitate contacting us!

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